antibody npnt goat polyclonal r d systems  (R&D Systems)

Journal: PLoS ONE

Article Title: Endotoxin-induced acute lung injury in mice with postnatal deletion of nephronectin

doi: 10.1371/journal.pone.0268398

Figure Lengend Snippet: (A) qPCR analysis of Npnt mRNA levels in lung and kidneys from Cre- and Cre+ mice after 4 weeks on the tamoxifen diet followed by normal chow. Expression is shown relative to Cre-. Shown are the means (bars) ± SE. Immunofluorescence staining for NPNT (red) in Cre- (B) and Cre+ (C) lungs from mice fed a tamoxifen diet for 4 weeks followed by chow. Nuclei were stained with DAPI. The insets show the localization of NPNT in Cre- and reduced staining in Cre+ lung tissue. Representative hematoxylin and eosin staining of sections of lung from uninjured Cre- (D) and Cre+ (E) using the same time course of dietary treatment. Scale bar = 50 μm for all images.

Article Snippet: Sections were incubated overnight at 4°C with a goat anti-mouse NPNT polyclonal (R&D, catalog number AF4298) or IgG isotype control (Jackson Immunoresearch, catalog number 005-000-003) at 2 μg/ml in TBST containing 1% BSA.

Techniques: Expressing, Immunofluorescence, Staining

Journal: PLoS ONE

Article Title: Endotoxin-induced acute lung injury in mice with postnatal deletion of nephronectin

doi: 10.1371/journal.pone.0268398

Figure Lengend Snippet: (A) Schematic of the time course of LPS injury and sample acquisition. (B) mRNA levels of Npnt in lungs from Cre- mice were measured by qPCR and expression calculated relative to uninjured. qPCR was also used to determine if expression of integrin α8 ( Itga8 ) (C) or the NPNT homologue EGFL6 (D) is altered by LPS injury of Cre+ lungs. Gene expression was calculated relative to uninjured Cre-. Shown are the medians (bars) with 95% confidence intervals. Data were analyzed using either the Mann-Whitney or Kruskal-Wallis statistical test. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. (E-H) Immunofluorescence for NPNT at day 3 (E and F) and day 7 (G and H) post LPS in Cre- (E and G) and Cre+ (F and H) lung tissue. Nuclei were stained with DAPI (blue). Scale bar = 100 μm.

Article Snippet: Sections were incubated overnight at 4°C with a goat anti-mouse NPNT polyclonal (R&D, catalog number AF4298) or IgG isotype control (Jackson Immunoresearch, catalog number 005-000-003) at 2 μg/ml in TBST containing 1% BSA.

Techniques: Expressing, Gene Expression, MANN-WHITNEY, Immunofluorescence, Staining